Chromatin Immunoprecipitation Assay Covaris truChIPTM Tissue Chromatin Shearing Kit with SDS Shearing Buffer and truChIP Low Cell Chromatin Shearing Kit with SDS Shearing Buffer
نویسندگان
چکیده
Chromatin shearing is the first critical step to provide sensitive and reproducible results in chromatin immunoprecipitation (ChIP) experiments. Adaptive Focused Acoustics (AFA) acoustic technology provides precise control over mechanical shearing and thermal control during processing to deliver high quality chromatin for the most sensitive results, in a highly reproducible manner. Optimizing the sample preparation and chromatin shearing provides more chromatin available for the immunoprecipitation, preserves protein epitopes, protein-DNA interactions, and DNA quality. Hamid Khoja, Gary Smejkal, Anna Krowczynska, J.D. Herlihy — Covaris, Inc., Woburn, Massachusetts, USA www.covarisinc.com tr u C h IP Application Note
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